Reactive oxygen species fluorescence staining of the cerebral cortex and cerebral microvessels from brains of Apo E−/−
mice exposed to mixed vehicle emissions or filtered air. Representative dihydroethidium (ethidium, red) fluorescence staining of (frontal) cerebral cortex sections (A,B) and cerebral microvessels (D,E) from brains of Apo E−/− mice exposed to either (A,D) mixed vehicular engine exhaust (MVE, 100 μg PM/m3) or (B,E) filtered air (FA) for 6 hr/day, 30 days. A, B scale bar = 100 μm; D, E scale bar = 10 μm. Arrows indicate areas of increased DHE staining. C, F = graphical quantification of total fluorescence per unit area. n = 5-6 per group, 3 slides (6 section) per animal, 2 sites (areas) each, were used for analysis. *p < 0.050 compared to FA control.