Protein expression of claudin - 5 and occludin in cerebral microvessels from Apo E−/−
mice exposed to either mixed vehicle emissions or filtered air. Representative western blots of protein expression of claudin-5 and occludin from cerebral microvessels dissected from the superior aspect of the cerebrum of Apo E−/− mice exposed to either 100 μgPM/m3 of mixed vehicular emission (MVE) (n = 4 pooled samples of 2 animals each) or filtered air controls (FA) (n = 4 pooled samples of 2 animals each) for 6 hr/day, for 30 days; bottom row gel – β actin loading control. Graph shows densitometric quantification of blots. *p < 0.050 compared to FA control.