Figure 3
Cytotoxicity of CuO NP, CuO MP and CuCl 2 . Cytotoxicity was determined by colony forming ability (CFA). Logarithmically growing (A) A549 or (B) HeLa S3 cells were treated with CuO NP, CuO MP or CuCl2 for 24 h, trypsinized, counted and reseeded. Shown are mean values of at least 9 determinations + SD. Statistically different from control cells: *p < 0.05, **p < 0.01, ***p < 0.001 as determined by unifactorial analysis of variance (ANOVA) followed by Dunnett’s T3 test. 1 μg/mL are equal to 0.2 μg/cm2 and 50 μg/mL CuO are equal to 630 μM Cu2+ in case of complete dissolution.