Figure 7

p53 regulates CSC-like cells from SWCNT-exposed lung epithelial cells. (A) Western blot analysis of p53 protein (left) and tumor growth kinetics (right) of wild-type (WT) and p53-overexpressed BSW tumors. (B) Quantitative analysis of tumor spheres (left) and side population (right) in WT and p53-overexpressed BSW cells. (C) Cell invasion (left) and migration (right) assays of WT and p53-overexpressed BSW cells. Plots are relative invasion and migration level normalized to WT cells. (D) Analysis of stem cell surface markers CD24 and CD133 in WT and p53-overexpressed BSW cells using flow cytometry. Plots are relative fluorescence level normalized to WT cells. Data are means ± SD (n = 3). * p < 0.05 vs. WT cells. NS, not significant vs. BC cells.