Figure 2

Cytoviva imaging. A. The figure is an example of typical images from light and enhanced darkfield imaging of MWCNT in lungs and diaphragm. The light micrograph of H&E stained section demonstrates MWCNT (black fibers) in the alveolar interstitium of a MWCNT Air exposed animal 17 months following inhalation exposure. This micrograph shows an example of MWCNT present within the alveolar interstium. Light microscope image using 100x oil immersion objective. Magnification bar is 10 microns. B. The micrograph is an enhanced darkfield image from the lung of an animal 17 months following exposure to MWCNT. The central area of the micrograph shows a region of alveolar wall with numerous MWCNT fibers present. The MWCNT are bright white due to imaging of scattered light over a broad range of wavelengths by this nanomaterial. Lung tissue, which does not significantly scatter light, is brown-to-orange and airspaces are black. The enhanced darkfield microscope image was photographed using a 100x oil immersion objective. The magnification bar is 20 microns. C. An enhanced darkfield imagine showing MWCNT in the diaphragm. MWCNT (indicated by the upper two arrows) are bright white. Nuclei are brown-to-orange, muscle cells are green and red blood cells are yellow. The parietal pleural border is indicated by the arrow in the lower part of the figure. Enhanced darkfield microscope image using 100x oil immersion objective. Magnification bar is 20 microns.