Figure 1

Micrographs demonstrating AMG silver enhanced clustered gold nanoparticles in mouse after in vivo exposure. (a) 40 nm gold particles clustered in Kupffer cells of the liver from a pregnant mouse. (b) Section from a fetal liver taken from an embryo of the same animal. Note that the fetal tissue is completely void of staining. The pregnant animal was intravenously injected 40 nm gold particles and allowed to survive for 24 hours. Both sections were 3 micron Epon sections counterstained with toluidine blue. (c) Section from the liver of a pregnant mouse, which served as a control and was exposed to saline. The section is completely void of AMG staining; (d) Micrograph of nanogold particles in a spleen macrophage. The animal was treated intravenously with 40 nm gold particles 24 hours before being sacrificed. 3 micron Epon section counterstained with Toluidine blue. (e) Enhanced gold nanoparticles in macrophages of a mesenterial lymph node. The animal was injected 40 nm gold nanoparticles intraperitoneally and allowed to survive for 4 hours, 30 μm thick cryo section, counterstained with toluidine blue. (f) Micrograph of a mesenterial lymph node of a mouse which was exposed to saline intraperitoneally and served as control. Scalebars = 20 μm.