Figure 3

Iron nanoparticles induce cell permeability through microtubule remodeling in HMVECs. A, Iron nanoparticles induce microtubule remodeling and redistribution. HMVECs were grown on coverslips and serum-starved overnight. Iron nanoparticles (50 μg/ml) were used to stimulate the cells for 30 min. After exposure, the cells were fixed and stained for acetylated β-tubulin (red color) and VE-cadherin (green color). A Zeiss confocal microscope was applied to take the images. The bottom panels of each image are overlays of two different stains. The size of the scale bar is 20 μm. B, A time course of iron nanoparticle-induced microtubule stabilization. HMVECs were grown to a confluent monolayer and serum-starved overnight. The cells were exposed to 50 μg/ml iron nanoparticles for different periods of time as indicated. After exposure, the cells were lysed. The lysates were resolved on SDS-PAGE gel, and anti-acetylated α-tubulin antibody was used to detect acetylated α-tubulin. C, The monolayer HMVECs were pretreated with either 100 nM paclitaxel or 10 nM nocodazole for one hour as indicated, followed by exposure to 50 μg/ml iron nanoparticles for 30 min. The cells were fixed and stained for acetylated α-tubulin (red color) and VE-cadherin (green color). A Zeiss confocal microscope was applied to take the images. Each image is an overlay of two different stains. The size of the scale bar is 20 μm.