Figure 3

DEP and PAMP modulate protein expression of Scavenger Receptors in MDDC. MDDC were cultivated with TLR2, -3 and -4 ligands respectively Pam₃CSK₄ (10 μg/ml), poly(I:C) (10 μg/ml) and LPS (1 μg/ml), associated or not with DEP (10 μg/ml). A-B Dendritic cells activated during 6 h (A) and 24 h (B) were labelled for CD36, CXCL16, LOX-1 and SR-B1, and analyzed by flow cytometry. Data are expressed as the mean ± SEM from 3 to 7 independent experiments. +: p < 0.05; ++: p < 0.01 compared with unstimulated cells. ✻: p < 0,05 compared with TLR-treated cells. C-D Flow cytometry histograms of a representative experiment. MDDC were cultivated for 6 h (C) and 24 h (D) with DEP (blue line), with TLR2 ligand (Pam3CSK4, red line), and with both stimuli (green line) as compared with cells in medium alone (black line).