Table 2 The fold change and MS results of the differently expressed proteins
From: SiO2 nanoparticles induce cytotoxicity and protein expression alteration in HaCaT cells
Spot no. | Protein ID | Fold change (dosage:10 μg/mL) | NCBI database no. | Mr (Da)/PI | Protein score | Coverage rate* % | ||
|---|---|---|---|---|---|---|---|---|
| Â | Â | Micro-sized SiO 2 | 30-nm SiO 2 | 15-nm SiO 2 | Â | Â | Â | Â |
Oxidative stress-associated proteins | ||||||||
717 | peroxiredoxin 6 | -1.21 | -.199 | -2.11 | gi|4758638 | 25019.2/6 | 413 | 58 |
796 | peroxiredoxin 1 | -1.20 | -1.44 | -1.63 | gi|4505591 | 22096.3/8.2 | 397 | 54 |
806 | peroxiredoxin 2 isoform a | -1.33 | -1.62 | -1.88 | gi|32189392 | 21878.2/5.66 | 375 | 47 |
1019 | thioredoxin | -1.22 | -2.59 | -2.44 | gi|50592994 | 11729.7/4.82 | 161 | 45 |
779 | GSTP1 | -1.31 | -1.84 | -1.95 | gi|4699783 | 23325/4.55 | 253 | 52 |
Cytoskeleton-associated proteins | ||||||||
416 | gelsolin-like capping protein (CAPG) | -1.46 | -1.92 | -2 | gi|63252913 | 38474.5/5.82 | 168 | 29 |
248 | keratin 8 | -1.37 | -1.31 | -1.35 | gi|33875698 | 55787.2/5.62 | 640 | 53 |
760 | keratin 19 | 1.89 | 4.35 | 4.06 | gi|34783124 | 45587/5.11 | 402 | 51 |
217 | keratin 4 (408 AA) | -- | 1.31 | 1.20 | gi|34073 | 57249.8/6.25 | 289 | 44 |
Molecular chaperones | ||||||||
797 | cyclophilin | -1.45 | -1.90 | -1.87 | gi|4758950 | 23727.5/9.42 | 310 | 67 |
922 | PPIA | -- | -1.45 | -1.37 | gi|48145531 | 17986.9/7.68 | 71 | 44 |
Metabolism-associated proteins | ||||||||
307 | enolase 1 variant | -1.30 | -1.92 | -2.17 | gi|62896593 | 47111.3/7.01 | 265 | 50 |
688 | phosphoglycerate mutase (PGAM) | -1.21 | -1.86 | -1.79 | gi|89035672 | 28831.8/6.67 | 240 | 43 |
132 | ATP5A1 | -1.27 | -1.79 | -1.79 | gi|4757810 | 59713.6/9.16 | 444 | 50 |
682 | Chain, CCarbonic Anhydrase Ii | -1.48 | -2.34 | -2.36 | gi|443135 | 28718.7/6.63 | 338 | 59 |
Apoptosis and tumor-associated proteins | ||||||||
436 | maspin | -1.36 | -1.60 | -1.70 | gi|4505789 | 42111.4/5.72 | 219 | 33 |