Figure 2
Effects of quartz and supernatants from quartz-treated macrophages on epithelial NF-κB pathway activation A. Immunocytochemistry of RelA nuclear translocation in RLE cells treated with supernatants from quartz-treated macrophages (Q-MS), untreated control macrophages (c-MS), or after direct treatment with quartz (Q). These are compared with untreated control cells (c). Cells were treated for 30 min with Q-MS and c-MS or quartz. Orange staining indicates the presence of the RelA subunit of the NF-κB complex. Original magnification: 400×. B. DNA binding activity of nuclear extracts from RLE cells treated with 40 μg/cm2 quartz for 24 h (Q), or with supernatants from untreated (c-MS) or quartz-treated (Q-MS) macrophages for 30, 60 or 120 min. Data are expressed as percentage of non-treated controls. *P < 0.05 and **P < 0.01 vs. control (100%).