Figure 4
From: A 3-dimensional in vitro model of epithelioid granulomas induced by high aspect ratio nanomaterials
Light microscopic morphology and kinetics of macrophage aggregation in 2D and 3D cultures. BMDM were exposed to 0.5 μg/ml (0.38 μg/cm2) of particulates. Formation of stable cellular aggregates was evaluated at 3 and 14 days post-exposure. Macrophages were stained with May-Grünwald-Giemsa as described in Materials and Methods.