Figure 1

Characterization and uptake of MAF02 particles. The fly ash was resuspended in filtered air, deposited on a Nuclepore™ membrane and analyzed by SEM (A). The bar indicates 2 μm. The aerosol was additionally measured by PCS-2000 light scattering spectrometer (B). The graph is representative of three measurements. For TEM analysis RAW264.7 cells were left untreated (C) or exposed to 50 μg/ml MAF02 particles (10.4 μg/cm²) for 1 h (D), 2.5 h (E) or 5 h (F). Representative images of three different experiments are shown. Arrows indicate MAF02 particles in agglomerates of different sizes.