Figure 2
From: Regulation of the arachidonic acid mobilization in macrophages by combustion-derived particles
MAF02 reduces the viability of human MDM and RAW264.7 macrophages and induces intracellular ROS in dependence of dose. (A) For testing viability human MDM and RAW264.7 macrophages were treated with MAF02 particles at 10, 50, 100 and 200 μg/ml (6.3, 31.3, 62.5 and 125 μg/m2) for 20 hours. The viability was analyzed using the WST-1 assay and results are given in percentage of untreated controls. (B) For studying ROS formation macrophages were treated with the same particle concentrations for 3 h and subsequently loaded with H2DCF. Induction of ROS was detected by fluorescence measurement of DCF and changes in fluorescence intensity are expressed as percentage of untreated controls. Results are the mean ± s.e.m. of three independent experiments, each carried out in triplicate.