Skip to main content

Table 3 EC 50 values (μg/ml) obtained from different assays after 24 h exposure of NR8383 and Caco-2 cells to positively charged PNP 45 [[4]] and PNP 90

From: Cytotoxicity and cellular uptake of tri-block copolymer nanoparticles with different size and surface characteristics

Assay

Parameter

Reference figure

Cell line

PNP 90 -NH 2 (90 ± 5 nm)

PNP 45 -NH 2 (45 ± 5 nm)

MTT

Cell viability

3

NR8383

55

31

   

Caco-2

68

54

PI

Phagocytosis

4

NR8383

80

64

DCFH-DA

Intracellular ROS production

5

NR8383

23

13

   

Caco-2

33

21

Mitochondrial membrane potential (ΔΨm)

Mitochondrial membrane potential

6

NR8383

5

2

   

Caco-2

6

3

ATP

Cellular ATP content

7

NR8383

26

14

   

Caco-2

62

36

TNF-α

TNF-α release

8

NR8383

37

25

   

Caco-2

63

32

Back to article page

Particle and Fibre Toxicology

ISSN: 1743-8977

Contact us