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Table 2 Microarray results

From: Intestinal toxicity evaluation of TiO2 degraded surface-treated nanoparticles: a combined physico-chemical and toxicogenomics approach in caco-2 cells

Microarray Analyses Total number of genes Number of detected genes* Number of genes up- or down- expressed
(>1.5 fold change )
Number of genes
up- or down- expressed
(pvalue < 0.05)**
% of genes altered out of detected spots
CTRL 2 vs CTRL 1 41000 23425 970 5 0,021%
Ti-Lite vs CTRL 1 41000 23828 419 1 0,004%
Ti-Lite DL vs CTRL 2 41000 22989 845 2 0,009%
Ti-Lite DA vs CTRL 41000 19928 824 0 0%
H2O2 vs CTRL 41000 28900 14651 9307 32,2%
  1. Caco-2 cells were cultured and differentiated for 21 days. The cells were exposed for 72 h at 10 μg/mL of TiO2 STNPs native or degraded. After mRNA extraction, labeled cDNA (Cy3) was hybridized to Agilent oligomicroarray (41 000 genes). The number of genes detected above a raw intensity threshold (background + 2SD) was compared for each type of STNPs exposed versus unexposed cells (*). From the remaining spots, we selected those with fluorescence ratios (representing STNPs exposed versus unexposed samples) greater than 1.5-fold change cutoff. Then we determined the statistical significance of the changes with pvalue ≤ 0.05 using a student t-test statistical analysis (n = 4) and performing a Benjamini and Hochberg false discovery rate multiple testing correction (**) using Genespring software. At the end of this analysis, we obtained lists of genes which were significantly induced or repressed after exposure to STNPs.