Figure 6
Assessment of cell viability and microarray analysis of NHBE cells in response to silica particle exposure. Effects of silica particles on NHBE cells after 24 h exposures. (A) Cell viability assessed by the Trypan blue exclusion assay (N = 3 in 2 independent experiments) *P < 0.05 compared to controls. NS = Not Significant for comparison of all exposure groups. (B) Total number of gene changes ≥ 2.0-fold vs. controls induced by silica particle exposure for 24 h. (C-E) Gene ontology analysis of NHBE exposed to (B) Cristobalite at 15 × 106μm2/cm2, (C) 75 × 106μm2/cm2 and (D) amorphous silica at 75 × 106μm2/cm2. Positive values represent number of genes up-regulated (Black bars) and negative values represent number of gene down-regulated (white bars) (N = 3).