Figure 1From: Diesel exhaust particulate induces pulmonary and systemic inflammation in rats without impairing endothelial function ex vivo or in vivo Instillation of diesel exhaust particles (DEP) causes transient pulmonary inflammation. (a) Representative photomicrograph of a cytocentrifuge slide prepared from bronchoalveolar lavage fluid (BALF) collected from a DEP-instilled animal 6 h after instillation. Neutrophils (open arrow) and alveolar macrophages (black arrow) containing particulate (purple arrow) are apparent. Diff-Quick™ staining, ×400 magnification. BALF was analysed for (b) total cell count, (c) neutrophils, (d) alveolar macrophages, (e) total protein (bicinchonic acid assay) and (f) interleukin-6 (IL-6). Non-instilled (open columns), saline-instilled (solid columns) and DEP-instilled (hatched columns) animals 6 and 24 h after instillation. Results are expressed as mean ± SEM (n = 4-8; data for non-instilled groups were pooled, n = 11) ***P < 0.001 DEP versus saline; two-way ANOVA followed by Bonferroni post-hoc test.Back to article page