Central IKKβ inhibition prevents air pollution mediated peripheral inflammation and exaggeration of type II diabetes

Table 2 Energy metabolism response to intracerebroventricular infusion of IMD 0354 in KKay mice exposed to PM _2.5

Phase	Measured index	Method	FA-CON vs PM-CON			PM-CON vs PM-IMD
Phase	Measured index	Method	Estimate	SE	p-value	Estimate	SE	p-value
Light + Dark	O₂	Avg	−370.927	149.129	0.032	331.662	167.254	0.061
		RM	−371.022	59.968	0.000	335.500	64.236	0.000
	CO₂	Avg	−333.440	127.020	0.025	347.582	181.272	0.070
		RM	−332.649	62.399	0.000	351.053	76.737	0.000
	RER	Avg	0.000	0.009	0.985	0.011	0.016	0.473
		RM	0.000	0.006	0.989	0.011	0.009	0.205
	Heat	Avg	−1.826	0.722	0.030	1.694	0.855	0.061
		RM	−1.825	0.303	0.000	1.712	0.336	0.000
Dark	O₂	Avg	−346.732	185.106	0.076	340.550	185.106	0.081
		RM	−340.496	82.903	0.000	336.695	82.903	0.000
	CO₂	Avg	−372.246	209.234	0.090	414.139	209.234	0.062
		RM	−359.378	97.723	0.000	406.893	97.723	0.000
	RER	Avg	−0.016	0.017	0.360	0.028	0.017	0.117
		RM	−0.012	0.010	0.248	0.026	0.010	0.012
	Heat	Avg	−1.781	0.958	0.078	1.809	0.958	0.073
		RM	−1.743	0.433	0.000	1.786	0.433	0.000

O₂ consumption, CO₂ production, RER and heat production of mice measured by indirect calorimetry over a 24 hrs period (from 10:00 am to 10:00 am the next day) after 5-week PM_2.5 exposure. n = 6.
Avg = analysis performed based on mice averages over cycles. Covariates in the model include PM exposure, treatment group, and the interaction between PM and treatment group.
RM = analysis with repeated measurements. The correlation structure within mice was assumed to be autoregressive-1. Covariates in the model include PM, treatment group, PM x treatment interaction, and time.
Mean model using averages over cycles (Avg): E (Y) = b0 + b1PM + b2 Trt + b3 PM*Trt.
Mean model using repeated measures data (RM): E (Y) = b0 + b1PM + b2 Trt + b3 PM*Trt + b4cycle.
Covariance structure: autoregressive-1.

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