Figure 3
From: Silica-induced NLRP3 inflammasome activation in vitro and in rat lungs
Silica polymorphs augment release of NLRP3-associated inflammatory and fibrotic mediators as well as alarmins from lung epithelial cells and differentiated macrophages, which is dependent on surface area reactivity. Investigation of markers for inflammasome activation in BEAS-2B (upper panels) and THP-1 (lower panels) cells following exposure to cristobalite, native and PVNO coated DQ12 silica for 24 h at a dose of 150 x 106 μm2/cm2. ELISAs were performed on concentrated medium for detection of secreted IL-1β (A), bFGF (B) and HMGB1 (C). Data are presented as mean ± SEM with * p-value <0.05 compared to UC, £ p-value < 0.05 compared to SIL150 and # p-value <0.05 compared to DQ12 silica exposure.