Figure 1

Pulmonary inflammatory endpoints measured in Bmal1^+/+and Bmal1^-/-mice, 24 hours (T1) and 8 weeks (T2) after the last exposure to multi-walled carbon nanotubes or zinc oxide nanoparticles. Total BAL cells (A, E), BAL neutrophils (B, F), pulmonary IL-1β concentration (C, G) and pulmonary KC concentration (D, H) were measured in each mouse. White bars depict measurements at T1, grey bars depict measurements at T2. Significant differences between the experimental groups due to the NP administration are depicted by letters (a, b and c). The effects after NP administration is indicated with letters depicted above the groups. A different letter indicates a significant effect of this group compared to the control of the same time point, within the same genotype. Time-effects are indicated by Δ. Δp < 0.05 compared to the same experimental group of T1. ΔΔp < 0.01 compared to the same experimental group of T1. When a symbol is placed in the middle of the figure, the time effects (Δ) are observed in all experimental groups.