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Table 2 IC 50 values related to exposure to Ag ENMs for Relative growth activity (RGA) and Plating efficiency (PE) assays in A549 cells (Time of exposure 2, 24, 48 h)

From: Is the toxic potential of nanosilver dependent on its size?

   Ag ENMs 50 nm Ag ENMs 80 nm Ag ENM 200 nm
Relative growth activity     
[μg/cm2] T = 2 h 16.8 ± 1.71 24.42 ± 1.82 23.68 ± 1.51
T = 24 h 23.68 ± 11.42 37.53 ± 17.58 36.37 ± 12.84
T = 48 h 15.94 ± 1.33 26.09 ± 11.63 21.71 ± 7.41
[ENMs × 1011/cm2] T = 2 h 6.57 ± 0.6 2.51 ± 0.19 0.46 ± 0.03
T = 24 h 9.26 ± 4.47 3.86 ± 1.81 0.75 ± 0.25
T = 48 h 6.23 ± 0.52 2.69 ± 1.2 0.42 ± 0.14
[ENMs cm2/cm2] T = 2 h 2.3 ± 0.23 1.85 ± 0.13 0.61 ± 0.53
T = 24 h 3.24 ± 1.33 2.84 ± 1.33 1.41 ± 0.5
T = 48 h 2.18 ± 0.18 1.97 ± 0.88 0.84 ± 0.29
Plating efficiency     
[μg/cm2] T = 2 h 35.58 ± 13.7 - -
T = 24 h 31.65 ± 15.81 31.65 ± 15.81 -
T = 48 h 17.83 ± 4.5 14.25 ± 1.94 -
[ENMs × 1011/cm2] T = 2 h 13.91 ± 5.35 - -
T = 24 h 12.37 ± 6.18 3.66 ± 0.41 -
T = 48 h 6.97 ± 1.77 1.48 ± 0.2 -
[ENMs cm2/cm2] T = 2 h 4.87 ± 1.89 - -
T = 24 h 4.37 ± 2.17 2.69 ± 0.3 -
T = 48 h 2.44 ± 0.62 1.08 ± 0.15 -
  1. -IC50 could not be estimated. IC50 values were calculated for 3 different concentration characterization units: number of ENMs (ENMs × 1011/cm2), surface area of ENMs (ENMs cm2/cm2) and mass of ENMs (μg/cm2) per exposure surface.
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