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Fig. 6 | Particle and Fibre Toxicology

Fig. 6

From: Differential bioreactivity of neutral, cationic and anionic polystyrene nanoparticles with cells from the human alveolar compartment: robust response of alveolar type 1 epithelial cells

Fig. 6

Transmission electron micrographs of TT1, AT2 and MAC following exposure to 50 nm UNP, CNP and ANP for 4 h. The data are presented as TEM images (a-l) and quantitatively as bar graphs (m-o). Following exposure to 50 μg/ml 50 nm NPs, TT1 and MAC internalised all three types of NPs, (m, o, arrows in b, c, d, j, k, l), whereas much lower numbers of AT2 cells internalised NPs (n). UNP and CNP were observed adhering to AT2 cell membranes (arrows in f-g). Once internalised, all types of NPs were observed mainly in endosomal structures. Percentage cell uptake is presented in m-o (*p < 0.05, **p < 0.001, n = 3, total 90 cells analysed). The reduction in mean fluorescence intensity (MFI) of Lysotracker® probe (p) indicates the lysosomal disruption following 4 h exposure of TT1 cells to ANPs (**p < 0.001, n = 3)

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