Fig. 3

Induction of systemic immune responses by Dp + nSP30 agglomerates in PBS. a–c Plasma levels of Dp-specific (a) IgE, (b) IgG, and (c) IgG1 and IgG2a as analyzed by ELISA at 24 h after final topical treatment of NC/Nga mice with Dp alone or Dp + nSP30 in PBS. (D) Numbers of Dp-specific IFN-γ- and IL-4-producing splenocytes after re-stimulation with 100 μg mL⁻¹ Dp, as determined by using ELISPOT assays specific for each cytokine. Data are given as means ± SEMs (n = 5–12). **P < 0.01 vs. Dp-alone group