Effects of silver nanoparticles and ions on a co-culture model for the gastrointestinal epithelium

Table 1 Overview of particle characteristics. Main characteristics of the Ag particles studied and the ions released in cell culture medium containing 10 % FBS after 24 h at the highest concentration at which the cells were exposed to (100 mg/L)

Particles	Primary size (nm)	Hydrodynamic diameter (nm)	Z potential (mV)	Soluble Ag released (μg/L)
Ag 20 nm	20	129	-12.8	<10 (0.01 %)
Ag 200 nm	200	308	-13.9	< 10 (0.01 %)

The Z potential in MilliQ water was -14.2 and -11 for Ag 20 and 200 nm, respectively while the values in DMEM without phenol red and FBS were +3.5 (Ag 20 nm) and +0.5 mV (Ag 200 nm)

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