Fig. 2
From: Poorly soluble cobalt oxide particles trigger genotoxicity via multiple pathways
Co3O4P do not exert cytotoxicity in BEAS-2B cells. CellTiter-Blue® and CellTiTer-Glo® showed that Co3O4P induced a slight and not statistically significant cytotoxicity in BEAS-2B cells. Either analyzing the mitochondrial activity (CellTiter-Blue®) or the ATP content (CellTiter-Glo®) after 24 h of exposure, the cellular viability was reduced by only about 20 % at the highest concentration tested (100 μg mL-1 cobalt). Differently, the cytotoxicity of CoCl2 was dose related and at 100 μg mL-1 the viability of BEAS-2B was reduced by about 80–85 %. IC50 CellTiter-Blue®: 31.30 ± 3.07 μg mL-1 cobalt; IC50 CellTiter-Glo®: 24.04 ± 3.75 μg mL-1 cobalt. Data are presented as mean % ± SEM of two independent experiments in triplicate. Statistical significance was evaluated by one-way ANOVA with Holm-Sidak post-hoc test: *p < 0.05; **p < 0.01; ***p < 0.001