Fig. 5
From: Poorly soluble cobalt oxide particles trigger genotoxicity via multiple pathways
Evaluation of double strand breaks in BEAS-2B cells exposed to cobalt using γ-H2Ax staining. The graphical representation of the phosphorylation of the histone H2Ax as (a) mean number of foci per cell indicates that CoCl2 is slightly more genotoxic that Co3O4P. This conclusion is further supported by the mean number of cells that developed foci after exposure to 10 μg mL-1 cobalt, (b), which clearly shows how CoCl2 induced more foci formation than Co3O4P. C pos (0.10 μg mL-1 MMC) was highly significant compared with C neg. Data are presented as (a) mean number of foci per cell ± SEM and (b) mean number of cells with a given number of foci (two independent experiments in duplicate). Statistical significance was evaluated by one-way ANOVA with Holm-Sidak post-hoc test: ***p < 0.001