μg mL-1
| CBPI | % cytostasis | Apoptotic index |
|---|
C neg | 1.61 ± 0.05 | 0.00 ± 0.00 | 0.25 ± 0.06 |
C pos | 1.34 ± 0.08* | 44.01 ± 4.04*** | 0.99 ± 0.06*** |
LB-3 | 1.64 ± 0.01 | 0.00 ± 2.43 | 0.17 ± 0.07 |
Co3O4P | 1.25 | 1.62 ± 0.02 | 0.00 ± 2.57 | 0.21 ± 0.06 |
2.50 | 1.63 ± 0.02 | 0.00 ± 2.57 | 0.34 ± 0.16 |
5 | 1.59 ± 0.06 | 2.84 ± 3.67 | 1.61 ± 0.06*** |
10 | 1.48 ± 0.11 | 20.96 ± 5.57 | 0.57 ± 0.11 |
20 | 1.48 ± 0.12 | 20.96 ± 6.02 | 0.60 ± 0.13 |
100 | 1.39 ± 0.10 | 36.68 ± 6.49** | 0.62 ± 0.02* |
CoCl2
| 1.25 | 1.62 ± 0.01 | 0.00 ± 2.43 | 0.06 ± 0.06 |
2.50 | 1.55 ± 0.03 | 9.43 ± 2.58 | 0.39 ± 0.15 |
5 | 1.49 ± 0.01 | 19.34 ± 1.97** | 1.02 ± 0.06*** |
10 | 1.34 ± 0.05* | 44.01 ± 2.73*** | 0.60 ± 0.07* |
| | 20 | 1.20 ± 0.00** | 67.07 ± 0.78*** | 0.10 ± 0.06 |
- The cytostatic effects induced in BEAS-2B after 24 h exposure to Co3O4P and CoCl2 were evaluated by the cytokinesis-block proliferation index (CBPI). Compared with the C neg, only CoCl2 at 10–20 μg mL-1 cobalt induced a slightly significant reduction of CBPI. By contrast, Co3O4P and their control, LB-3, did not exert any cytostatic effect on BEAS-2B cells. The % cytostasis confirmed the toxicity of CoCl2, but highlighted the significance of the exposure to the highest Co3O4P concentration tested (100 μg mL-1 cobalt). Differently, the cytotoxicity evaluated by scoring the apoptotic index showed that Co3O4P and CoCl2 exerted significant effects at 5 μg mL-1, whereas a mild apoptosis was observed after treatment with Co3O4P and CoCl2 (10 μg mL-1 cobalt). The positive control, MMC (0.1 μg mL-1), was cytostatic and cytotoxic. Data are expressed as mean value ± SEM of two independent experiments, each in duplicate. Statistically significant differences from the C neg were determined by one-way ANOVA followed by Holm-Sidak method for comparisons between groups: *p < 0.05, **p < 0.01 and ***p < 0.001
