Fig. 5

Localization of silver in the liver evaluated by confocal microscopy. Representative images of liver (scale bar = 20 μm), from vehicle- (control), AgAc, and CT- and PVP-coated AgNP (10 nm, 40 nm, 100 nm)-treated mice. Aggregates of silver (green) are found within the cytoplasm of Iba-1+ Kupffer cells (red) (white arrows) only in AgNP-treated mice. Rarely, small numbers of silver aggregates are found outside Kupffer cells (yellow arrow), most likely in the cytoplasm of endothelial cells or hepatocytes. Nuclei are counterstained with DAPI (cyan)