Fig. 7
Rescue effects of NAC against treatment with AgNP-110 at different concentrations on HUVEC cells. In NAC treated groups, cells were pretreated with 5 mM NAC for 1 h. a Cell viability from CCK-8 assay. b The intracellular ROS level. c Cells were incubated for 1 h with AgNP-110 or AgNO3 containing 1 μg/mL of Ag, inter-endothelial junction stained with VE-cadherin antibody. The scale bar represents 20 μm. The cells treated with 7.5 mg/mL H2O2 was set as positive control. The * represents significant difference between control group and AgNP-110-treated group (*: p < 0.05, **: p < 0.01), the # represents significant difference between groups with and without NAC pretreatment (#: p < 0.05, ##: p < 0.01)