Table 1 PCR Primers
Gene Name | Acronym | Function | Forward primer | Reverse primer |
|---|---|---|---|---|
glyceraldehyde-3-phosphate dehydrogenase | GAPDH | a house-keeping gene encoding enzyme for the oxidative phosphorylation of glyceraldehyde-3-phosphate | TGAACGGGAAGCTCACTGG | TCCACCACCCTGTTGCTGTA |
pro-opiomelanocortin-alpha | POMC | a polypeptide hormone precursor involved in energy homeostasis | GCCCTCCTGCTTCAGACCTC | CGTTGCCAGGAAACACGG |
neuropeptide Y | NPY | a neuropeptide that regualtes food intake | TACCCCTCCAAGCCGGACAA | TTTCATTTCCCATCACCACATG |
agouti related neuropeptide | AgRP | a neuropeptide that regulates feeding behavior | CGGAGGTGCTAGATCCACAGA | AGGACTCGTGCAGCCTTACAC |
interleukine-1beta | IL-1b | a pro-inflammatory cytokine | ACGGACCCCAAAAGATGAAG | TTCTCCACAGCCACAATGAG |
interleukine-6 | IL-6 | a pro-inflammatory cytokine | ATCCAGTTGCCTTCTTGGGACTGA | TAAGCCTCCGACTTGTGAAGTGGT |
tumor necrosis factor alpha | TNFα | a pro-inflammatory cytokine | TTCCGAATTCACTGGAGCCTCGAA | TGCACCTCAGGGAAGAATCTGGAA |
suppressor of cytokine signaling 3 | SOCS3 | cytokine-inducible negative regulators of cytokine signaling | GCGGGCACCTTTCTTATCC | TCCCCGACTGGGTCTTGAC |
Leptin | Â | an adipokine involved in regulation of body weight | GGGTAATACTTAAACAGTGACC | CTATCTGAAAATAAAAACTTCATG |
Adiponectin | Â | an adipokine involved in regulation of fatty acid catabolism and glucose levels | AGGGAGAGAAAGGAGATGCAG | CTTTCCTGCCAGGGGTTC |
fatty acid synthase | FAS | the synthesis of palmitate from acetyl-CoA and malonyl-CoA | TGCTCCCAGCTGCAGGC | GCCCGGTAGCTCTGGGTGTA |
peroxisome proliferator activated receptor gamma | PPARγ | a regulator of adipocyte differentiation. | TCGCTGATGCACTGCCTATG | GAGAGGTCCACAGAGCTGATT |
sterol regulatory element binding transcription factor 1c | SREBP-1c | a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is involved in sterol biosynthesis | GATGTGCGAACTGGACACAG | CATAGGGGGCGTCAAACAG |
Acetyl-CoA carboxylase | ACC | A biotin-containing enzyme which catalyzes the rate-limiting step in fatty acid synthesis. | GCCGTGGGGAAGGAAAAGT | CTCCTGGTTGATGCTCGACA |
PPARG coactivator 1 alpha | PGCα | a transcriptional coactivator involved in energy metabolism. | GAGAATGAGGCAAACTTGCTAGCG | TGCATGGTTCTGAGTGCTAAGACC |
estrogen receptor 1 (alpha) | ER | a member of the nuclear hormone family of intracellular receptors | ACCATTGACAAGAACCGGAG | CCTGAAGCACCCATTTCATT |
CCAAT/enhancer binding protein alpha | CEBP | a transcription factor involved in body weight homeostasis. | CTGCGGGGTTGTTGATGT | ATGCTCGAAACGGAAAAGGT |
Preadipocyte factor 1 | PREF1 | Inhibits Adipogenesis | AGTGCGAAACCTGGGTGTC | GCCTCCTTGTTGAAAGTGGTCA |