Skip to main content

Table 3 Medium conditions for mono- and cocultures. Unless further mentioned, mono- and cocultures were handled in the absence of antibiotic and antimycotic agents. All media contained Glutamax instead of L-glutamine

From: Endothelial responses of the alveolar barrier in vitro in a dose-controlled exposure to diesel exhaust particulate matter

Monocultures

Cell Line

Medium

Serum

Supplements

A549

Dulbecco’s Modified Eagle’s Medium (DMEM)

10% (v/v) Fetal Bovine Serum Gold

 

THP-1

Roswell Park Memorial Institute (RPMI) 1640

25 mM HEPES;

50 μM β-mercaptoethanol

HMC-1

Iscove’s Modified Dulbecco’s Medium (IMDM)

25 mM HEPES; 1,2 mM α-thioglycerol

EA.hy 926

Dulbecco’s Modified Eagle’s Medium (DMEM)

25 mM HEPES

Tetraculture

75% HEPES-buffered DMEM; 15% RPMI; 10% IMDM

1% (v/v) Fetal Bovine Serum Gold

25 mM HEPES