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Table 3 Medium conditions for mono- and cocultures. Unless further mentioned, mono- and cocultures were handled in the absence of antibiotic and antimycotic agents. All media contained Glutamax instead of L-glutamine

From: Endothelial responses of the alveolar barrier in vitro in a dose-controlled exposure to diesel exhaust particulate matter

Monocultures
Cell Line Medium Serum Supplements
A549 Dulbecco’s Modified Eagle’s Medium (DMEM) 10% (v/v) Fetal Bovine Serum Gold  
THP-1 Roswell Park Memorial Institute (RPMI) 1640 25 mM HEPES; 50 μM β-mercaptoethanol
HMC-1 Iscove’s Modified Dulbecco’s Medium (IMDM) 25 mM HEPES; 1,2 mM α-thioglycerol
EA.hy 926 Dulbecco’s Modified Eagle’s Medium (DMEM) 25 mM HEPES
Tetraculture
75% HEPES-buffered DMEM; 15% RPMI; 10% IMDM 1% (v/v) Fetal Bovine Serum Gold 25 mM HEPES