Fig. 5

Fibroblast accumulation. WT and Opn KO mice were exposed to DM or 40 μg MWCNTs for 7 or 28 days. (a) Immunofluorescence of fibroblast marker Hsp47 on mouse lung sections. Green indicates positive staining, and blue indicates nuclear staining (scale bar: 20 μm). (b) Immunofluorescence of fibroblast marker Vimentin on mouse lung sections. Red indicates positive staining, and blue indicates nuclear staining (scale bar: 20 μm). In (a) and (b), relative intensity of positive staining was measured and presented as mean ± SD (n = 4). (c) Immunoblotting of fibroblast marker FSP1 (n = 2). Actin was used as the loading control