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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Prenatal tobacco smoke exposure predisposes offspring mice to exacerbated allergic airway inflammation associated with altered innate effector function

Fig. 3

Prenatal ETS exposure exacerbates allergen-induced accumulation of inflammatory cells in the lungs of the offspring. The effect of prenatal ETS or filtered air exposure on Siglec-F+ eosinophilis, CD4+ T cells and monocyte-derived dendritic cells (DC) accumulation in the lungs was investigated in 12-week old offspring. Mice (6 per group) were intranasally challenged with HDM allergen or PBS (control). Bronchoalveolar lavage fluid (BALF) was collected, and lung mononuclear cell (LMC) isolated by collagenase dispersion of lung tissue for analysis. The frequency of a CD11b+Gr1Siglec-F+ eosinophils (after gating on CD11b+ and F4/80 cells), b CD3+CD4+ T cells in the BALF, and c CD11b+CD11c+MHC-IIbright dendritic cells (DC) in the LMC was determined using multicolor flow cytometry. Data are representative of at least two independent experiments

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