Fig. 2

Acute inflammation in response to pulmonary MWCNT instillation is IL-1 dependent. C57BL/6 and IL-1R1 KO mice were intratracheally administered 162 μg of MWCNTs, and samples were collected 24 h and 28 days later. a Graphical representation of the key event targeted by IL-1R1 KO. b IL-1α and IL-1β were measured in BAL fluid of WT animals. The horizontal lines indicate limits of detection; IL-1 α – below 2.0 pg and IL-1 β – 0.04 pg. c The number of total cells, mononuclear cells, and neutrophils was determined from cytospin slides generated from BAL fluid and cell concentration measurements from BAL. d The inflammatory cytokines CXCL1, IL-6, and IL-12p40 were measured in BAL fluid. e Distribution of MWCNTs was determined by hyperspectral imaging in H&E stained histology samples. Data represent mean ± SEM. n = 4–5. Statistical analysis was performed using two-way ANOVA. *p < 0.05, NS = not significant, †statistical interaction with p < 0.05, and NI = no statistical interaction