Fig. 4

IL-1 deficiency differentially affects more MWCNT-induced fibrotic genes at 24 h compared to 28 days. RNA was isolated from the lung tissue of MWCNT-administered mice 24 h and 28 days post-exposure. a The number of DEGs is visualized by a bar chart and the number of significant (at least a 1.5 fold change and an FDR adjusted p < 0.05) DEGs between WT and IL-1R1 KO mice is indicated for both time points. b Venn analysis was used to visualize the degree of overlap between increasing and decreasing DEGs at the 24 h and 28 day time points. c A heat map visualizing all of the DEGs involved in inflammation and fibrosis is shown. The genes are ordered based on the difference in expression between WT and IL-1R1 KO mice