Fig. 3

O-PMs induced ROS production and ICAM-1 expression in A549 cells through AKT/STAT3/p65 phosphorylation. a-b The effects of O-PMs treatment on the phosphorylation of (a) ERK, p38, JNK (b) AKT, p65, and STAT3 in A549 cells. A549 cells were exposed to 0, 25, 50 or 100 μg/ml O-PMs for 24 h. Cell lysates were analyzed by Western blot with antibodies against p-ERK, t-ERK, p-JNK, t-JNK, p-p38, t-p38, p-AKT, t-AKT, p-p65, t-p65, p-STAT3, and t-STAT3. c The effects of NAC treatment on the phosphorylation of AKT, p65 and STAT3 in O-PMs-treated A549 cells by Western blot. A549 cells were pretreated with 5 mM of NAC for 1 h and then treated with 100 μg/ml O-PMs for 24 h. *p < 0.05 vs. Con. d-e A549 cells were pretreated with BAY11–7082 (10 μM), LY294002 (10 μM), SP600125 (10 μM), SB203580 (10 μM), or Stattic (10 μM) for 1 h and then treated with 100 μg/ml O-PMs for 24 h. ICAM-1 protein in cell lysates was measured by Western blot (D) and the ICAM-1 surface expression was detected by flow cytometry (E). *p < 0.05 vs. Con. †p < 0.05 vs. O-PMs