Fig. 6

PMs-induced ICAM-1 and IL-6 expression in lung tissues of WT mice. WT and IL-6 KO mice were intratracheal instillation with 200–350 mg/mouse PMs for 7 and 14 days. Mice were euthanized, and plasma and lung tissues were collected at the determined time. a-c The levels of ICAM-1and IL-6 expression in lung tissues of WT and IL-6 KO mice were examined by immunohistochemical staining (a-b) and Western blot (c). The reaction product is indicated by arrows. The boxed regions were enlarged and shown in the right panel. Bar = 50 mu. d-e The levels of sICAM-1 and IL-6 in plasma were measured by ELISA. Data are expressed as mean ± SEM. (n = 6 for each group). *p < 0.05 vs. WT or IL-6 KO at Day 0, respectively. †p < 0.05 vs. WT at Day 7 or Day 14. WT mice (n = 6) were treated NAC (3–5 mg/mouse, 150 mg/kg body weight) before PM intratracheal instillation (200–350 mg/mouse). f The levels of ICAM-1and IL-6 expression in lung tissues were examined by immunohistochemical staining. g The expression of ICAM-1 and IL-6 as well as the phosphorylation of AKT, p65 and STAT3 in lung tissues were examined by Western blot. h-i The levels of sICAM-1 and IL-6 in plasma were measured by ELISA. *p < 0.05 vs WT at Day 0. †p < 0.05 vs Day 7