Fig. 2

Effects of MWCNT on the DNFB induced CHS ear swelling responses after 24 h. Mice were sensitized with 30 μl of 0.05% DNFB in a 4:1 acetone/olive oil vehicle on the lower dorsum on day 0. On day 5, the mice were challenged with either vehicle or 0.2% DNFB. The right ears (black bars) are controls that were not treated with MWCNT. The left ears (gray bars) were treated with either pristine MWCNT, MWCNT Low-COOH, MWCNT High-COOH, or MWCNT High-COOH 0.45 μm filtrate. The DNFB increased ear swelling significantly, compared to all vehicle treated mice. Only ears treated with DNFB and MWCNT High-COOH displayed significantly increased swelling responses, compared to DNFB only. Graphs represent changes in mean ear swelling after 24 h (+/− SEM), N = 5. A Student’s T-test was used to analyze all data presented here, since comparisons were between corresponding right and left ears. Significance is defined at a p-value < 0.05, and # indicates significance between groups