Fig. 6
From: Multi-walled carbon nanotube oxidation dependent keratinocyte cytotoxicity and skin inflammation
MWCNT High-COOH nanoparticles augment skin expression of inflammatory cytokines. Mice were sensitized with 30 μl 0.05% DNFB in a 4:1 acetone/olive oil vehicle on the abdomen on day 0. On day 5 the mice were collared to prevent grooming and challenged with 50 μl of either vehicle, 5 μg MWCNT, 0.2% DNFB, or 0.2% DNFB with 5 μg MWCNT on a 5 cm2 area of dorsal skin for 24 h. A 1 cm2 piece of skin was homogenized and the cytokine protein expression levels were analyzed by Luminex assay. The graphs represent the mean (+/− SEM), N = 6. All data was analyzed by two-way ANOVA except for MCP-1, which was analyzed by one-way ANOVA, due to some data being below the limit of detection. Significance is defined at a p-value < 0.05. * indicates significance compared to control, # indicate significance within groups