Mitsui-7, heat-treated, and nitrogen-doped multi-walled carbon nanotubes elicit genotoxicity in human lung epithelial cells

Table 3 Aneuploidy in SAEC cell exposed to MWCNT material

Treatment		Total Aneuploidy (%)	Chromosome1 (%)	Gain (%)	Loss (%)	Chromosome 4 (%)	Gain (%)	Loss (%)
Control		9 ± 4	7 ± 3	4 ± 2	3 ± 3	6 ± 3	4 ± 1	3 ± 2
V₂O₅		41 ± 11*	33 ± 13*	13 ± 4	20 ± 11	19 ± 9	9 ± 3	10 ± 6
MWCNT-7 (μg/mL)	0.024	59 ± 13*⁺	41 ± 5*	10 ± 6	31 ± 11^¥	37 ± 19*	19 ± 24	18 ± 7
	0.24	59 ± 13*	45 ± 8*⁺	13 ± 10	32 ± 16^¥	35 ± 17*	13 ± 13	21 ± 8
	2.4	61 ± 7*	45 ± 16*	7 ± 2	39 ± 18^¥	40 ± 13*	9 ± 8	31 ± 13^¥
	24	67 ± 3*	49 ± 7*	9 ± 2	40 ± 6^¥	48 ± 3*⁺	8 ± 5	40 ± 8^¥
MWCNT-HT (μg/mL)	0.024	44 ± 14*⁺	32 ± 5*	18 ± 10	14 ± 6	29 ± 13*	14 ± 8	15 ± 7
	0.24	45 ± 13*	31 ± 1*⁺	14 ± 3	17 ± 3	29 ± 16*	19 ± 16	10 ± 0
	2.4	51 ± 6*	38 ± 7*	14 ± 11	24 ± 13	29 ± 10*	15 ± 9	15 ± 7
	24	61 ± 7*	45 ± 6*	8 ± 6	36 ± 8^¥	38 ± 8*	10 ± 7	28 ± 14^¥
MWCNT-ND (μg/mL)	0.024	48 ± 18*	36 ± 8*	14 ± 8	21 ± 5	30 ± 15*	16 ± 9	15 ± 7
	0.24	46 ± 10*	33 ± 4*	9 ± 5	23 ± 7^¥	29 ± 13*	17 ± 12	12 ± 2
	2.4	51 ± 6*	42 ± 7*	11 ± 10	31 ± 11^¥	28 ± 13*	15 ± 5	13 ± 10
	24	56 ± 14*	42 ± 8*	16 ± 13	26 ± 8	34 ± 15*⁺	16 ± 15	18 ± 4

This table represents the percentage of aneuploidy in SAEC cells exposed to each MWCNT material and 0.316 μg/mL V₂O₅, positive control, for 24 h. Percentage of total aneuploidy is representative of aneuploidy of chromosomes 1 and 4 combined and based on the total number of cells analyzed. Percentage of aneuploidy was also separated by chromosome and a loss or gain of either chromosome 1 or 4 was also recorded as a percentage of total cells. * indicates significantly different from control, p < 0.05. ¥ indicates significantly different from counterpart MWCNT materials, p < 0.05. + indicates significantly different from each other, within treatment group, p < 0.05. ± SD

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