Fig. 4

HIF-1α drives lung inflammation induced by LCO. C57BL/6Jrj mice were treated with an oro-pharyngeal aspiration of NaCl (control), 0.5 or 2 mg LCO or crystalline silica (Sil). Mice received i.p. injections of 0.5 mg/kg bw/d chetomin or vehicle (saline solution with 10% DMSO) at day − 1, 1 and 2 and were enthanized at day 3. Alveolar inflammatory cell infiltration and neutrophils were assessed in the BAL (a, b). IL-1β (c) and IL-6 (d) were measured in BALF; Lung sections of mice exposed to 2 mg particles were stained with HE (magnification 10x and 200x) (e). *P < 0.05, **P < 0.01 and ***P < 0.001 (t-test between chetomin - and + mice for each condition, N = 1, n = 5, means ± SEM)