Fig. 1
Concentration-dependent release of cytokines after Si10 and Min-U-Sil exposure in 3D tri-culture. The 3D tri-culture consisted of THP-1 macrophages and A549 cells in the apical compartment and Ea.hy 926 endothelial cells in the basolateral compartment as described in Material and Methods. The figure shows cytokine levels in the apical and the basolateral compartment after 20 h exposure to Si10 (0–48 μg/cm2) and Min-U-Sil (0–192 μg/cm2). Cytokine levels were determined by ELISA, and data represent the mean +/− SEM of 5 independent experiments. *Statistical significant difference from control, #statistical significant difference from basolateral side, P < 0.05