Fig. 6

PARP activation mediates SiNPs-induced blockage of autophagic flux in BEAS-2B. a Western blotting analysis of the levels of LC3 and SQSTM1 in BEAS-2B cells treated with different concentrations of SiNPs for 24 h. b Representative confocal images showing GFP-LC3-RFP in cells treated with SiNPs (100 μg/mL) in the absence or presence of PJ34 (10 μM) at 24 h. Scale bar = 10 μm. c-d Mean number of yellow puncta (autophagosomes) and red puncta (autolysosomes) in each cell from 30 cells for each condition. e-f. Western blotting analysis of the levels of LC3 (e) and SQSTM1 (f) in BEAS-2B cells under control condition or after treated with SiNPs (100 μg/mL) in the absence or presence of PJ34 (10 μM), from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared to control cells, and ^#P < 0.05, ^##P < 0.01 compared to cells treated with SiNPs alone