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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Jejunal villus absorption and paracellular tight junction permeability are major routes for early intestinal uptake of food-grade TiO2 particles: an in vivo and ex vivo study in mice

Fig. 2

Confocal and TEM localization of TiO2 particles in jejunal villi, 4 h after E171 oral administration. a-f In the confocal analysis, the TiO2 particles are apparent in green (and overlined by green arrows), the tissue auto-fluorescence in red and the nucleus labelled by Dapi in blue for some images a-d. a Absorption in jejunal villus showing its organization, the limit between the epithelial monolayer (Epith) and the lamina propria (LP) is indicated by a yellow dashed line. The GC are indicated by white arrows and one is circled by a white dashed line. A TiO2 particle is visible in or near a GC (a, d), in the lamina propria (b) or in the epithelium (d). g, h TEM associated to EDX analysis: TiO2 particles are detected in the mucin-rich vesicles of a GC (g) or in the basal side of an enterocyte (h). i Intestinal permeability measurement using Ussing chambers in the jejunum from controls (Ctrl) and TiO2 treated animals (TiO2). The jejunum was recovered 4 h after a single oral dose of E171, longitudinally opened and transferred in a Ussing chamber. The transfer of FFS from the luminal to the serosal side of the jejunum is measured (FFS flow expressed in ng/ml) and the transepithelial resistance of the tissue (TER in Ohms x cm2 of tissue). Bars = 10 μm in confocal microscopy, and 1 μm or 200 nm in TEM

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Particle and Fibre Toxicology

ISSN: 1743-8977

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