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Fig. 6 | Particle and Fibre Toxicology

Fig. 6

From: Jejunal villus absorption and paracellular tight junction permeability are major routes for early intestinal uptake of food-grade TiO2 particles: an in vivo and ex vivo study in mice

Fig. 6

Confocal detection of TiO2 and of epithelium surfaces in the jejunum after in vivo or ex vivo exposure to E171, and comparative analysis of ex vivo absorption of TiO2 particles and of polystyrene beads. a, b: after TiO2 exposure, in vivo (4 h) (a) or ex vivo (30 min) (b), GC and apical surface of epithelial cells were labelled in red with the FM-1-43-FX dye; TiO2 is visible in green (arrows) and the nuclei stained with DAPI (blue). BBM = Brush border membrane of enterocytes (Ent), L = lumen, LP = lamina propria. (c, d, e): Jejunal loops were incubated ex vivo without (Ctrl) or with TiO2 (E171 300 μg/ml), alone or in the presence of the TJ blocker TAP (c) or the endocytosis blockers EIAP, Pitstop or MβCD (e). Results were expressed in number of particles (P)/(1000 x mm3 of tissue). After incubation the luminal medium was recovered to measure the release of LDH from tissues, and was expressed in activity/mg of tissue (d). In c ***: significantly different for TiO2 alone versus Ctrl or TiO2 + TAP (p < 0.001). In e) ***: significantly different from Crtl (p < 0.001), and NS: not significant versus TiO2 alone. f Quantification of ex vivo absorption of TiO2 or polystyrene beads in jejunal villi and PP; ***: significantly different between villi and PP (p < 0.001)

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Particle and Fibre Toxicology

ISSN: 1743-8977

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