Fig. 4

O-PMs affected the expression of fibronectin, E-cadherin and vimentin via the ETS-1 pathway. a A549 cells were transfected with 50 nM ETS-1 siRNA and incubated for 24 h. Cells transfected with or without ETS-1 siRNA were treated with 100 μg/mL O-PMs for 24 h. The expression levels of fibronectin, E-cadherin and vimentin were measured by Western blot. b Co-immunoprecipitation of ETS-1 and fibronectin in A549 cells. Cell lysates were precipitated with anti-ETS-1 antibody-conjugated agarose beads, and then blotted with anti-ETS-1 and anti-fibronectin antibodies. Fibronectin precipitation was detected in the precipitate of the ETS-1 immune complex. c The effect of ETS-1 siRNA on the migratory abilities of O-PMs-treated A549 cells was determined by a wound healing assay. A representative image of each group at different time points after wound formation is shown. The red lines represent the wound boundaries. The percentage of the wound area is expressed as the mean ± SEM, n = 3. Bar = 100 μm. *p < 0.05 compared to control (Con) cells; †p < 0.05 relative to O-PMs-treated cells