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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: LiCoO2 particles used in Li-ion batteries induce primary mutagenicity in lung cells via their capacity to generate hydroxyl radicals

Fig. 4

LCO particles induce DNA strand breaks and DNA oxidative lesions in lung epithelial cells in vitro. Rat lung epithelial cells (RLE, 15600 cells/cm2) were exposed to culture medium (control, CTL), WC-Co, LCO or LTO and cytotoxicity was assessed after 24 h by the WST-1 assay (a). Alkaline comet assay, with or without oxidative DNA lesion repair enzyme (hOGG1), was performed 24 h after exposure to particles (b). *P < 0.05, **P < 0.01 and ***P < 0.001 (t-test between alkaline and alkaline + hOGG1 conditions). Bars represent means ± SEM (N = 4 with n = 4 for the WST-1 assay, N = 4 for the alkaline comet assay performed without hOGG1 and N = 2 for the alkaline comet assay performed with hOGG1, n = 2). ND = not determined

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