Fig. 1
From: Airborne particulate matter (PM2.5) triggers ocular hypertension and glaucoma through pyroptosis
a Particle size distribution analyzed by dynamic light-scattering. PM2.5 suspension was analyzed by dynamic light-scattering for three times and the average sizes of PM is 876 nm. b PM2.5 exposure induced ocular hypertension in C57BL/6 mice. PM2.5 suspension was applied topically to the mouse eyes. The intraocular pressure (IOP) was measured without anesthesia by rebound tonometry. IOP was elevated gradually in PM2.5-treated mouse eyes compared with the controls (n ≥ 6, *P < 0.05, paired sample t-test or Mann-Whitney U test). c PM2.5 increased the expressions of NLRP3 inflammasome-related proteins in mouse eye outflow tissues. Relative protein expressions of NLRP3 (n = 3), caspase-1 (n = 4), GSDMD (n = 3) and IL-1β (n = 3) were determined by western blot and showed in A-D. *P < 0.05, independent sample t-test. Date are represented as the mean ± standard deviation. C, PBS-treated control; E, PM2.5 exposure. d Mock PM2.5 particles penetrated the mouse cornea and deposited in intraocular tissues. Mock PM2.5 particles with diameters ranging from 10 nm to 500 nm passed through the cornea, entered the anterior chamber and finally deposited on the outflow tissue. The dotted line shows the outline of the iris. Epi, cornea epithelium; end, cornea endothelium; CB, ciliary body