Fig. 3
From: Airborne particulate matter (PM2.5) triggers ocular hypertension and glaucoma through pyroptosis
Drug interventions on PM2.5 induced toxicity. a NAC or VX-765 pretreated for 2 h improved HTM cell viability in cells exposed to 100 μg/mL PM2.5 for 48 h (n = 5 cell lines, *P < 0.05, one-way ANOVA, compared with the control; #P < 0.05, one-way ANOVA, Compare with PM2.5 100 μg/mL). Date are represented as the mean ± standard deviation. b, c NAC and VX-765 inhibited PM2.5 induced NLRP3 inflammasome activation. Relative protein expressions of NLRP3 (n = 3), caspase-1 (n = 3), GSDMD (n = 3) and IL-1β (n = 5) were determined by western blot (B) and ELISA (C). *P < 0.05, one-way ANOVA, compared with the control; #p < 0.05, one-way ANOVA, compare with PM2.5 100 μg/mL. Ctrl, PBS-treated control; PM2.5, PM2.5 100 μg/mL; NAC + PM2.5, NAC 3 mM pretreated for 2 h + PM2.5 100 μg/mL; VX-765 + PM2.5, VX-765 100 uM pretreated for 2 h + PM2.5100 μg/mL. Date are represented as the mean ± standard deviation. d, e Immunofluorescence examination confirmed that NAC and VX-765 inhibited PM2.5 triggered NLRP3 axis activation in HTM cells. Representative immunofluorescence images of the punctate staining of NLRP3 (D) and caspase-1 (E) in HTM cells treated with the vehicle control or PM2.5 100 μg/mL for 48 h, pretreated with or without NAC 3 mM or VX-765 100 μM for 2 h. Blue, DAPI; red, NLRP3 (D) or caspase-1 (E). Magnification, 20X. f. NAC and VX-765 inhibited PM2.5 induced HTM cell contraction. NAC 3 mM or VX-765 100 μM pretreated for 2 h resulted in a strong inhibition of PM2.5 induced HTM contraction. Control, n = 3; PM2.5, n = 4; NAC + PM2.5, n = 4; VX-765 + PM2.5, n = 4. *P < 0.05, one-way ANOVA, compared with the control; #P < 0.05, one-way ANOVA, compared with PM2.5 100 μg/mL. Date are represented as the mean ± standard error of mean