Table 2 Primers used for RT-PCR
From: Airborne particulate matter (PM2.5) triggers ocular hypertension and glaucoma through pyroptosis
Gene | Primer |
|---|---|
NLRP3 | Forward: 5′-GCACTTGCTGGACCATCCTC-3’ |
Reverse: 5′-GTCCAGTGCACACGATCCAG-3’ | |
Caspase-1 | Forward: 5′-AAGACCCGAGCTTTGATTGACTC-3’ |
Reverse: 5′-AAATCTCTGCCGACTTTTGTTTCC-3’ | |
IL-1β | Forward: 5′-TATTACAGTGGCAATGAGG-3’ |
Reverse: 5′-ATGAAGGGAAAGAAGGTG-3’ | |
β-actin | Forward: 5′-CCCTGGACTTCGAGCAAGAG − 3’ |
Reverse: 5′-TCACACTTCATGATGGAGTTG-3’ |